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Accueil > Recherche > Plateformes > SpICy : SpectroImageries et Cytométrie > Service NanoIR

NanoIR1 platform

The nanoIR1 is designed with a bottom-up laser illumination (in total reflection). The major constraint is the necessity to deposit the sample on ZnSe prims or specific CaF2 windows.

Bottom-up illumination

Two options of IR laser sources :

1. OPO source 4000-1000 cm-1 : non-resonant mode. Sensitivity limit : 50-100 nm size. Spectral resolution 6-8 cm-1

2. QCL source, 1820-1520 cm-1 : resonant enhanced mode. Sensitivity limit : 20 nm. Spectral resolution < 1 cm-1.

Main advantages :
- the laser illumination is very well controlled to enable consistent measurements across the full spectrum
- the polarization of the light can be rotated to investigate polarization effects in materials
- optional liquid environment is available for FLUID AFM-IR
- Ideal for Eucaryotic cells/ polymer/ tissue sectioning
- Biomolecules aggregates or fibrils (bigger than 20nm)

Constraints :
-  Sample thickness must be less than 1 um on ZnSe prism but any size on CaF2 windows.
-  Sample thickness usually limits the resolution.
-  Works in AFM contact mode requiring good sample to prism adhesion (ZnSe or CaF2)