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Nanospectroscopie Infrarouge pour la localisation subcellulaire de biopolymères

Permanents : Alexandre Dazzi (PR), Ariane Deniset-Besseau (MC), Delphine Onidas (MC).

When AFM met IR : Monitoring Biopolymers in Micro-organisms at the Subcellular Scale by Infrared Nanospectroscopy

Research activity

Recently near-field techniques play a fundamental role in Nanoscience microscopy. Two different ways exist to make infrared studies with near-field techniques : optical techniques measuring the transmitted signal coming from the nano-object or photothermal approachs using thermometer to link temperature to absorption measurements. Considering the limitations of the optical techniques, we have developed in our team an innovative photothermal technique called AFM-IR. The AFM-IR (nanoIRTM) is a user-friendly benchtop technique that enables infrared spectroscopy with a spatial resolution well below conventional optical diffraction limits. It acquires IR absorption imaging spectrally resolved with lateral resolution down to 100 nm.

In our team we use this technique to study the production and localization of biopolymers in micro-organisms. Our different projects are performed in close collaboration with microbiologists and biophysicists. Now, the nanoIR setup is a facility proposed to external researcher looking for infrared spectroscopy and imaging analysis at nanometer scale (lien plateforme).

Proposition de thèse : / PhD proposal : Rolando Rebois, Jérémie Mathurin

Review papers / PhD manuscripts
- Thèse soutenue le //2010 par Céline Mayet

Selected publications :

Visible-light active conducting polymer nanostructures with superior photocatalytic activity OPEN 

Srabanti Ghosh · Natalie Amoin Kouame · Samy Remita · Laurence Ramos · Fabrice Goubard · Pierre-Henri Aubert · Alexandre Dazzi · Ariane Deniset-Besseau · Hynd Remita 
[Show abstract] 
Full-text · Article · Dec 2015 · Scientific Reports

Conducting Polymer Nanofibers of Controlled Diameter Synthesized in Hexagonal Mesophases 

ghosh srabanti · laurence ramos · samy remita · alexandre dazzi · ariane deniset · patricia beaunier · fabrice goubard · aubert pierre-henry · hynd remita 
[Show abstract] 
Full-text · Article · Nov 2015 · New Journal of Chemistry

Conducting polymer nanostructures for photocatalysis under visible light 

Srabanti Ghosh · Natalie A Kouamé · Laurence Ramos · Samy Remita · Alexandre Dazzi · Ariane Deniset-Besseau · Patricia Beaunier · Fabrice Goubard · Pierre-Henri Aubert · Hynd Remita 

Monitoring TriAcylGlycerols Accumulation by Atomic Force Microscopy Based Infrared Spectroscopy in Streptomyces Species for Biodiesel Applications 

Ariane Deniset-Besseau · Craig B. Prater ‡ · Marie-Joëlle Virolle § · Alexandre Dazzi † 
[Show abstract] 
Full-text · Article · Jan 2014 · Journal of Physical Chemistry Letters
PEDOT nanostructures synthesized in hexagonal mesophases 

Srabanti Ghosh · Hynd Remita · Laurence Ramos · Ariane Deniset-Besseau · Patricia Beaunier · Fabrice Goubard · Pierre-Henri Aubert · Francois Brisset · Samy Remita 
[Show abstract] 
Full-text · Article · Jan 2014 · New Journal of Chemistry

Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques 

Sylvain Clède · François Lambert · Christophe Sandt · Slavka Kascakova · Miriam Unger · Etienne Harté · Marie-Aude Plamont · Rénette Saint-Fort · Ariane Deniset-Besseau · Zoher Gueroui · Carol Hirschmugl · Sophie Lecomte · Alexandre Dazzi · Anne Vessières · Clotilde Policar 
[Show abstract] 
Full-text · Article · Jul 2013 · The Analyst

Minimising contributions from scattering in infrared spectra by means of an integrating sphere 

Alexandre Dazzi · Ariane Deniset-Besseau · Peter Lasch 
[Show abstract] 
No preview · Article · Jun 2013 · The Analyst

Radiation chemistry as an alternative way for the synthesis of PEDOT conducting Polymers under “soft” Conditions 

Youssef Lattach · Ariane Deniset-Besseau · Jean-Michel Guigner · Samy Remita 
[Show abstract] 
Full-text · Article · Jan 2013 · Radiation Physics and Chemistry

Analysis of bacterial polyhydroxybutyrate production by multimodal nanoimaging 

Céline Mayet · Ariane Deniset-Besseau · Rui Prazeres · Jean-Michel Ortega · Alexandre Dazzi 
[Show abstract] 
No preview · Article · May 2012 · Biotechnology advances