Instituto de Catálisis y Petroleoquímica, CSIC, Madrid
Mardi 4 décembre à 11h (salle Magat) :
“Functional anchoring of proteins on modified surfaces : from bacterial cytoskeleton to redox proteins”
Résumé
I will present recent work developed in my lab in which we have addressed the study of different membrane-associated proteins. We have used Quartz Crystal Microbalance (QCM) and Atomic force Microscopy (AFM) to characterize the structure and dynamic behavior of membrane-associated bacterial cytoskeletal protein FtsZ. This GTP-dependent self-organizing protein plays a central role in bacterial cell division. The high-resolution information obtained has been used in multiscale theoretical models that propose a force generation mechanism. I will also present recent work on orienting and immobilizing different membrane-associated redox and respiratory proteins on gold electrodes. We monitor enzymatic activity and the accompanying proton gradient formed across a supported lipid membrane. We use a combination of surface modification techniques to construct a modified electrode in which hydrogen consumption is coupled to ATP synthesis.
Références
- Pablo Mateos-Gil, Pedro Tarazona, Marisela Vélez ; “Bacterial Cell Division : Modeling FtsZ Assembly and Force Generation from Single Filament Experimental Data”, FEMS Microbiology Reviews
- Gutiérrez-Sanz, O., Natale, P., Márquez, I., Marques, M.C., Zacarias, S., Pita, M., Pereira, I.A.C., López-Montero,I., De Lacey,A.L. and Vélez, M. “ H2-Fueled ATP Synthesis on an Electrode : Mimicking Cellular Respiration” 2016, Angewandte Chemie , 55, 6216-6220
- Gutiérrez-Sanz,O, Tapia,C., Marques,M., Zacarias,S.,Vélez,M.,Pereira,I.,L. De Lacey,A. “Induction of a Proton Gradient across a Gold-Supported Biomimetic Membrane by Electroenzymatic H2 Oxidation” 2015, Angewandte Chemie Int Ed, 54, 2684–2687
